Summary
Catalog No:A7488
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - FN1 antibody (A7488)
ABclonal:Immunohistochemistry  - FN1 Antibody (A7488)
ABclonal:Immunohistochemistry  - FN1 Antibody (A7488)
ABclonal:Immunohistochemistry  - FN1 Antibody (A7488)

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Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-128 of human FN1 (NP_002017.1).
SequenceMLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGA
Gene ID2335
Swiss ProtP02751
SynonymsCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
Calculated MW71-73kDa/221-272kDa
Observed MW70kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse liver
Cellular LocationSecreted, extracellular matrix, extracellular space
Customer Validation

WB (Rat)

IF (Human)

FN1 Polyclonal Antibody images

ABclonal:Western blot - FN1 antibody (A7488)

Western blot - FN1 antibody (A7488)

Western blot analysis of extracts of mouse liver, using FN1 antibody (A7488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry  - FN1 Antibody (A7488)

Immunohistochemistry - FN1 Antibody (A7488)

Immunohistochemistry of paraffin-embedded rat brain using FN1 Antibody (A7488) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - FN1 Antibody (A7488)

Immunohistochemistry - FN1 Antibody (A7488)

Immunohistochemistry of paraffin-embedded mouse brain using FN1 Antibody (A7488) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - FN1 Antibody (A7488)

Immunohistochemistry - FN1 Antibody (A7488)

Immunohistochemistry of paraffin-embedded mouse kidney using FN1 Antibody (A7488) at dilution of 1:100 (40x lens).

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