Product Type > Antibodies > Secondary Antibodies

FITC-conjugated Goat anti-Mouse IgG (H+L) (AS001)

Publications (84) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Immunofluorescence - FITC-conjugated Goat anti-Mouse IgG (H+L) (AS001)

Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by a further incubation with FITC Goat Anti-Mouse IgG (H+L) (AS001, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - FITC-conjugated Goat anti-Mouse IgG (H+L) (AS001)

Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Mouse isotype control (blue) and non-staining control (Red).The secondary antibody used was FITC Goat Anti-Mouse IgG (H+L) (AS001) at 1:100.

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Overview

Product name FITC-conjugated Goat anti-Mouse IgG (H+L)
Catalog No. AS001
Host species Goat
Purification method Affinity purification
Isotype Fluorescein conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Mouse IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IF/ICC 1:100 - 1:500
  • FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Application key Immunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF (Rattus norvegicus, Homo sapiens, Mus musculus, Oryctolagus cuniculus, Danio rerio, Cyprinus gibelio, Sus scrofa, Grass carp, Saccharomyces cerevisiae, Spodoptera frugiperdaSus scrofa, Cyprinus carpio, Gallus gallus)

ADE (Homo sapiens)

FC (Sus scrofa)

IP (Mus musculus)

WB (Mus musculus, Escherichia coli, Sus scrofa, Homo sapiens)

IHC (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

FITC-conjugated Goat anti-Mouse IgG (H+L) images

ABclonal:Immunofluorescence - FITC-conjugated Goat anti-Mouse IgG (H+L) (AS001)}

Immunofluorescence - FITC-conjugated Goat anti-Mouse IgG (H+L) (AS001)

Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by a further incubation with FITC Goat Anti-Mouse IgG (H+L) (AS001, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - FITC-conjugated Goat anti-Mouse IgG (H+L) (AS001)}

Flow CytoMetry - FITC-conjugated Goat anti-Mouse IgG (H+L) (AS001)

Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Mouse isotype control (blue) and non-staining control (Red).The secondary antibody used was FITC Goat Anti-Mouse IgG (H+L) (AS001) at 1:100.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Mouse. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Mouse. (Distance between topics and target gene indicate popularity.) Goat Anti-Mouse

* Data provided by citexs.com, for reference only.

Publishing research using AS001? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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