Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Fbx32/FBOX32 Rabbit mAb (A11908)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Fbx32/FBOX32 Rabbit mAb (A11908)

Western blot analysis of extracts of various cell lines, using Fbx32/FBOX32 antibody (A11908).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Fbx32/FBOX32 Rabbit mAb
Catalog No. A11908
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant protein of human Fbx32/FBOX32
Sequence Email for sequence
Gene ID 114907
Swiss prot Q969P5
Synonyms Fbx32; MAFbx; Fbx32/FBOX32
Calculated MW 42kDa
Observed MW 37kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse muscle, Rat muscle
Cellular location Cytoplasm, Nucleus

Research Area

Fbx32/FBOX32 Rabbit mAb images

ABclonal:Western blot - Fbx32/FBOX32 Rabbit mAb (A11908)}

Western blot - Fbx32/FBOX32 Rabbit mAb (A11908)

Western blot analysis of extracts of various cell lines, using Fbx32/FBOX32 antibody (A11908).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on FBXO32. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FBXO32. (Distance between topics and target gene indicate popularity.) FBXO32

* Data provided by citexs.com, for reference only.

Publishing research using A11908? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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