Summary
Catalog No:A1324
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - EP300 antibody (A1324)

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Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

Immunogen information

ImmunogenRecombinant Protein of human EP300
SequenceEmail for sequence
Gene ID2033
Swiss ProtQ09472
SynonymsKAT3B, p300, RSTS2
Calculated MW264kDa
Observed MW17kDa/34kDa

Applications

ReactivityHuman
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRecombinant protein
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (SK-N-SH,SH-SY5Y,BE(2)C, Mouse)

EP300 Monoclonal Antibody images

ABclonal:Western blot - EP300 antibody (A1324)

Western blot - EP300 antibody (A1324)

Western blot analysis of extracts of Recombinant protein, using EP300 antibody (A1324).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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