Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (A11437)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (A11437)

Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (A11437).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb
Catalog No. A11437
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

Immunogen information

Immunogen Recombinant protein of human Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2))
Sequence Email for sequence
Gene ID 112398
Swiss prot Q96KS0
Synonyms EIT6; PHD1; EIT-6; HPH-1; HPH-3; HIFPH1; HIF-PH1; Prolyl hydroxylase PHD1 (EGLN2)
Calculated MW 44kDa
Observed MW 44kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, PC-12, NIH/3T3
Cellular location Nucleus

Research Area

Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb images

ABclonal:Western blot - Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (A11437)}

Western blot - Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (A11437)

Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (A11437).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on EGLN2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EGLN2. (Distance between topics and target gene indicate popularity.) EGLN2

* Data provided by citexs.com, for reference only.

Publishing research using A11437? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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