Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

EEF1E1 Mouse mAb (A4314)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - EEF1E1 Mouse mAb (A4314)

Western blot analysis of extracts of HepG2 cells, using EEF1E1 antibody (A4314).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name EEF1E1 Mouse mAb
Catalog No. A4314
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.

Immunogen information

Immunogen A synthetic peptide of human EEF1E1
Sequence Email for sequence
Gene ID 9521
Swiss prot O43324
Synonyms P18; AIMP3; EEF1E1
Calculated MW 20kDa
Observed MW 20kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:20 - 1:50
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunofluorescence    Flow Cytometry    
Positive samples HepG2
Cellular location Cytoplasm, Nucleus

EEF1E1 Mouse mAb images

ABclonal:Western blot - EEF1E1 Mouse mAb (A4314)}

Western blot - EEF1E1 Mouse mAb (A4314)

Western blot analysis of extracts of HepG2 cells, using EEF1E1 antibody (A4314).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on EEF1E1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EEF1E1. (Distance between topics and target gene indicate popularity.) EEF1E1

* Data provided by citexs.com, for reference only.

Publishing research using A4314? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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