Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

eEF1A1 Rabbit pAb (A0974)

Reviews (5) Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - eEF1A1 Rabbit pAb (A0974)

Western blot analysis of extracts of various cell lines, using eEF1A1 antibody (A0974) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - eEF1A1 Rabbit pAb (A0974)

Immunofluorescence analysis of U2OS cells using eEF1A1 Rabbit pAb (A0974) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for eEF1A1 Rabbit pAb(A0974)
Review
ABclonal: review for eEF1A1 Rabbit pAb(A0974)
Review
ABclonal: review for eEF1A1 Rabbit pAb(A0974)
Review
ABclonal: review for eEF1A1 Rabbit pAb(A0974)
Review
ABclonal: review for eEF1A1 Rabbit pAb(A0974)

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Overview

Product name eEF1A1 Rabbit pAb
Catalog No. A0974
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human eEF1A1 (NP_001393.1).
Sequence FEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
Gene ID 1915
Swiss prot P68104
Synonyms CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; EF1A1; LENG7; eEF1A-1; GRAF-1EF; EF1alpha1; eEF1A1
Calculated MW 50kDa
Observed MW 50kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T, MCF7, HeLa, Jurkat, HepG2, Mouse liver, Rat liver
Cellular location Cytoplasm, Nucleus, nucleolus
Customer validation

WB (293TOryza sativa L. spp. hsien Ting, Homo sapiens, Mus musculus)

IF (293T)

Documents

Certificate of Compliance

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Lot number

Research Area

eEF1A1 Rabbit pAb images

ABclonal:Western blot - eEF1A1 Rabbit pAb (A0974)}

Western blot - eEF1A1 Rabbit pAb (A0974)

Western blot analysis of extracts of various cell lines, using eEF1A1 antibody (A0974) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - eEF1A1 Rabbit pAb (A0974)}

Immunofluorescence - eEF1A1 Rabbit pAb (A0974)

Immunofluorescence analysis of U2OS cells using eEF1A1 Rabbit pAb (A0974) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on EEF1A1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EEF1A1. (Distance between topics and target gene indicate popularity.) EEF1A1

* Data provided by citexs.com, for reference only.

Publishing research using A0974? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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