Summary
Catalog No:A2356
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Dot-blot - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K4 antibody (A2356)
ABclonal:Chromatin immunoprecipitation - DiMethyl-Histone H3-K4 antibody (A2356)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

ImmunogenA synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss ProtQ16695
SynonymsH3.4, H3/g, H3FT, H3t
Calculated MW15kDa
Observed MW18kDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWB, IHC, IF, IP, ChIP, ChIPseq
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
CHIPseq 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, H3 protein
Cellular LocationChromosome, Nucleus
Customer Validation

WB (Penicillium oxalate, Human cells)

ChIP (Rice)

DiMethyl-Histone H3-K4 pAb images

ABclonal:Western blot - DiMethyl-Histone H3-K4 antibody (A2356)

Western blot - DiMethyl-Histone H3-K4 antibody (A2356)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody (A2356).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Dot-blot - DiMethyl-Histone H3-K4 antibody (A2356)

Dot-blot - DiMethyl-Histone H3-K4 antibody (A2356)

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody (A2356).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry - DiMethyl-Histone H3-K4 antibody (A2356)

Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K4 antibody (A2356) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K4 antibody (A2356)

Immunofluorescence - DiMethyl-Histone H3-K4 antibody (A2356)

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K4 antibody (A2356). Blue: DAPI for nuclear staining.
ABclonal:Chromatin immunoprecipitation - DiMethyl-Histone H3-K4 antibody (A2356)

Chromatin immunoprecipitation - DiMethyl-Histone H3-K4 antibody (A2356)

Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K4 antibody (A2356) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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