Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

DSG1 Rabbit pAb (A9812)

Review (1) Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - DSG1 Rabbit pAb (A9812)

Western blot analysis of extracts of various cell lines, using DSG1 antibody (A9812) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - DSG1 Rabbit pAb (A9812)

Immunofluorescence analysis of rat skin using DSG1 antibody (A9812) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DSG1 Rabbit pAb(A9812)

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Overview

Product name DSG1 Rabbit pAb
Catalog No. A9812
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 800-1049 of human DSG1 (NP_001933.2).
Sequence SPHFGTTTVISESTYPSGPGVLHPKPILDPLGYGNVTVTESYTTSDTLKPSVHVHDNRPASNVVVTERVVGPISGADLHGMLEMPDLRDGSNVIVTERVIAPSSSLPTSLTIHHPRESSNVVVTERVIQPTSGMIGSLSMHPELANAHNVIVTERVVSGAGVTGISGTTGISGGIGSSGLVGTSMGAGSGALSGAGISGGGIGLSSLGGTASIGHMRSSSDHHFNQTIGSASPSTARSRITKYSTVQYSK
Gene ID 1828
Swiss prot Q02413
Synonyms DG1; DSG; CDHF4; EPKHE; PPKS1; SPPK1; EPKHIA; DSG1
Calculated MW 114kDa
Observed MW 150kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples A-431, HeLa, 293T, BT-474
Cellular location Cell junction, Cell membrane, Single-pass type I membrane protein, desmosome
Customer validation

IF (Mouse keRatinocytes)

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

DSG1 Rabbit pAb images

ABclonal:Western blot - DSG1 Rabbit pAb (A9812)}

Western blot - DSG1 Rabbit pAb (A9812)

Western blot analysis of extracts of various cell lines, using DSG1 antibody (A9812) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - DSG1 Rabbit pAb (A9812)}

Immunofluorescence - DSG1 Rabbit pAb (A9812)

Immunofluorescence analysis of rat skin using DSG1 antibody (A9812) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on DSG1. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to DSG1. (Distance between topics and target gene indicate popularity.) DSG1

* Data provided by citexs.com, for reference only.

Publishing research using A9812? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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