Summary
Catalog No:A5495
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Dnmt1 antibody (A5495)
ABclonal:Immunohistochemistry  - DNMT1 antibody (A5495)
ABclonal:Immunohistochemistry  - DNMT1 antibody (A5495)
ABclonal:Immunohistochemistry  - DNMT1 antibody (A5495)

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Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).
SequenceMPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPK
Gene ID1786
Swiss ProtP26358
SynonymsADCADN, AIM, CXXC9, DNMT, HSN1E, m.HsaI, MCMT
Calculated MW144kDa/183kDa/184kDa
Observed MW240kDa

Applications

ReactivityHuman, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, SW480
Cellular LocationNucleus
Customer Validation

WB (Human hepatocellular carcinoma cell line, HUVEC, SMMC7721,BEL7404, Human cells, HEK293T, BEL-7402)

ChIP (Human)

IF (Human)

DNMT1 Polyclonal Antibody images

ABclonal:Western blot - Dnmt1 antibody (A5495)

Western blot - Dnmt1 antibody (A5495)

Western blot analysis of extracts of various cell lines, using Dnmt1 antibody (A5495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry  - DNMT1 antibody (A5495)

Immunohistochemistry - DNMT1 antibody (A5495)

Immunohistochemistry of paraffin-embedded rat spleen using DNMT1 antibody (A5495) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DNMT1 antibody (A5495)

Immunohistochemistry - DNMT1 antibody (A5495)

Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (A5495) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DNMT1 antibody (A5495)

Immunohistochemistry - DNMT1 antibody (A5495)

Immunohistochemistry of paraffin-embedded human lung cancer using DNMT1 antibody (A5495) at dilution of 1:100 (40x lens).

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