Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

DNMT1 Rabbit pAb (A5495)

Reviews (10) Publications (20) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Immunoprecipitation - DNMT1 Rabbit pAb (A5495)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg DNMT1 antibody (A5495). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A5495) at a dilution of 1:1000.

Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for DNMT1 Rabbit pAb(A5495)

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Overview

Product name DNMT1 Rabbit pAb
Catalog No. A5495
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 760-1109 of DNMT1 (NP_001370.1).
Sequence GDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNK
Gene ID 1786
Swiss prot P26358
Synonyms AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNMT1
Calculated MW 183kDa
Observed MW 200kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, SW480
Cellular location Nucleus
Customer validation

WB (Human hepatocellular carcinoma cell line, HUVEC, SMMC7721,BEL7404, Human cells, HEK293T, BEL-7402Homo sapiens, Homo sapiens, Mus musculus, Rattus norvegicus)

ChIP (Homo sapiens, Mus musculus)

IF (Leporidae, Homo sapiens, Rattus norvegicus)

IHC (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

DNMT1 Rabbit pAb images

ABclonal:Immunoprecipitation - DNMT1 Rabbit pAb (A5495)}

Immunoprecipitation - DNMT1 Rabbit pAb (A5495)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg DNMT1 antibody (A5495). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A5495) at a dilution of 1:1000.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on DNMT1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DNMT1. (Distance between topics and target gene indicate popularity.) DNMT1

* Data provided by citexs.com, for reference only.

Publishing research using A5495? Please let us know so that we can cite the reference in this datasheet.

Antibodies (9)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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