Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

DRP1 Rabbit pAb (A2586)

Reviews (3) Publications (50) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - DRP1 Rabbit pAb (A2586)

Western blot analysis of various lysates using DRP1 Rabbit pAb (A2586) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of DRP1 in paraffin-embedded rat brain using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of DRP1 in paraffin-embedded rat heart using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - DRP1 Rabbit pAb (A2586)

Immunofluorescence analysis of NIH/3T3 cells using DRP1 Rabbit pAb (A2586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DRP1 Rabbit pAb(A2586)
Review
ABclonal: review for DRP1 Rabbit pAb(A2586)
Review
ABclonal: review for DRP1 Rabbit pAb(A2586)

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Overview

Product name DRP1 Rabbit pAb
Catalog No. A2586
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 411-710 of human DRP1 (NP_036193.2).
Sequence VSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
Gene ID 10059
Swiss prot O00429
Synonyms DLP1; DRP1; DVLP; EMPF; OPA5; EMPF1; DYMPLE; HDYNIV
Calculated MW 82kDa
Observed MW 78kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse brain, Rat brain
Cellular location Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus, Membrane, Mitochondrion outer membrane, Peripheral membrane protein, Peroxisome, clathrin-coated pit, cytosol, secretory vesicle, synaptic vesicle membrane
Customer validation

WB (Human bone marrow cancer cell, HeLa, Mouse skeletal muscleRattus norvegicus, Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Yellow catfish)

IF (Rattus norvegicus, Homo sapiens, Mus musculus)

Co-IP (Homo sapiens)

IF/ICC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

DRP1 Rabbit pAb images

ABclonal:Western blot - DRP1 Rabbit pAb (A2586)}

Western blot - DRP1 Rabbit pAb (A2586)

Western blot analysis of various lysates using DRP1 Rabbit pAb (A2586) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)}

Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of DRP1 in paraffin-embedded rat brain using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)}

Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of DRP1 in paraffin-embedded rat heart using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - DRP1 Rabbit pAb (A2586)}

Immunofluorescence - DRP1 Rabbit pAb (A2586)

Immunofluorescence analysis of NIH/3T3 cells using DRP1 Rabbit pAb (A2586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on DNM1L. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DNM1L. (Distance between topics and target gene indicate popularity.) DNM1L

* Data provided by citexs.com, for reference only.

Publishing research using A2586? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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