Summary
Catalog No:A10841
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - DLG4 antibody (A10841)
ABclonal:Immunohistochemistry  - DLG4 antibody (A10841)
ABclonal:Immunohistochemistry  - DLG4 antibody (A10841)
ABclonal:Immunohistochemistry  - DLG4 antibody (A10841)
ABclonal:Immunofluorescence - DLG4 antibody (A10841)
ABclonal:Immunofluorescence - DLG4 antibody (A10841)

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Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenRecombinant protein of human DLG4
SequenceEmail for sequence
Gene ID1742
Swiss ProtP78352
SynonymsPSD95, SAP-90, SAP90
Calculated MW80kDa/85kDa
Observed MW80kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationCell junction, Cell membrane, Cell projection, Peripheral membrane protein, axon, postsynaptic cell membrane, postsynaptic density, synapse
Customer Validation

WB (Rat)

DLG4 Monoclonal Antibody images

ABclonal:Western blot - DLG4 antibody (A10841)

Western blot - DLG4 antibody (A10841)

Western blot analysis of extracts of various cell lines, using DLG4 antibody (A10841).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - DLG4 antibody (A10841)

Immunohistochemistry - DLG4 antibody (A10841)

Immunohistochemistry of paraffin-embedded mouse cerebellum using DLG4 antibody (A10841) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DLG4 antibody (A10841)

Immunohistochemistry - DLG4 antibody (A10841)

Immunohistochemistry of paraffin-embedded rat cerebellum using DLG4 antibody (A10841) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - DLG4 antibody (A10841)

Immunohistochemistry - DLG4 antibody (A10841)

Immunohistochemistry of paraffin-embedded rat brain using DLG4 antibody (A10841) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - DLG4 antibody (A10841)

Immunofluorescence - DLG4 antibody (A10841)

Immunofluorescence analysis of SH-SY-5Y cells using DLG4 antibody (A10841).
ABclonal:Immunofluorescence - DLG4 antibody (A10841)

Immunofluorescence - DLG4 antibody (A10841)

Immunofluorescence analysis of N2A cells using DLG4 antibody (A10841).

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