Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

DGCR8 Rabbit mAb (A11404)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - DGCR8 Rabbit mAb (A11404)

Western blot analysis of extracts of various cell lines, using DGCR8 antibody (A11404).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name DGCR8 Rabbit mAb
Catalog No. A11404
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant protein of human DGCR8
Sequence Email for sequence
Gene ID 54487
Swiss prot Q8WYQ5
Synonyms Gy1; pasha; DGCRK6; C22orf12; DGCR8
Calculated MW 86kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:500
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa, PC-12, NIH/3T3
Cellular location Nucleus, nucleolus

DGCR8 Rabbit mAb images

ABclonal:Western blot - DGCR8 Rabbit mAb (A11404)}

Western blot - DGCR8 Rabbit mAb (A11404)

Western blot analysis of extracts of various cell lines, using DGCR8 antibody (A11404).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on DGCR8. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DGCR8. (Distance between topics and target gene indicate popularity.) DGCR8

* Data provided by citexs.com, for reference only.

Publishing research using A11404? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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