Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

β-Catenin Rabbit pAb (A2064)

Reviews (4) Publications (10) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - β-Catenin Rabbit pAb (A2064)

Western blot analysis of various lysates using β-Catenin Rabbit pAb (A2064) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - β-Catenin Rabbit pAb (A2064)

Immunohistochemistry analysis of β-Catenin in paraffin-embedded rat pancreas using β-Catenin Rabbit pAb (A2064) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - β-Catenin Rabbit pAb (A2064)

Immunohistochemistry analysis of β-Catenin in paraffin-embedded human breast cancer using β-Catenin Rabbit pAb (A2064) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - β-Catenin Rabbit pAb (A2064)

Immunohistochemistry analysis of β-Catenin in paraffin-embedded mouse pancreas using β-Catenin Rabbit pAb (A2064) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - β-Catenin Rabbit pAb (A2064)

Immunofluorescence analysis of HeLa cells using β-Catenin Rabbit pAb (A2064) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for β-Catenin Rabbit pAb(A2064)
Review
ABclonal: review for β-Catenin Rabbit pAb(A2064)
Review
ABclonal: review for β-Catenin Rabbit pAb(A2064)
Review
ABclonal: review for β-Catenin Rabbit pAb(A2064)

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Overview

Product name β-Catenin Rabbit pAb
Catalog No. A2064
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic peptide of human beta Catenin
Sequence Email for sequence
Gene ID 1499
Swiss prot P35222
Synonyms EVR7; CTNNB; MRD19; NEDSDV; armadillo; β-Catenin
Calculated MW 85kDa
Observed MW 110kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples SW480, Mouse brain, Mouse lung, Rat brain
Cellular location Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

WB (Human chronic myeloid leukemia cellsHomo sapiens, Mus musculus, Homo sapiens, Rattus norvegicus)

IF (Human chronic myeloid leukemia cells)

IHC (Mouse intestines, Rat kidneyHomo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

β-Catenin Rabbit pAb images

ABclonal:Western blot - β-Catenin Rabbit pAb (A2064)}

Western blot - β-Catenin Rabbit pAb (A2064)

Western blot analysis of various lysates using β-Catenin Rabbit pAb (A2064) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - β-Catenin Rabbit pAb (A2064)}

Immunohistochemistry - β-Catenin Rabbit pAb (A2064)

Immunohistochemistry analysis of β-Catenin in paraffin-embedded rat pancreas using β-Catenin Rabbit pAb (A2064) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - β-Catenin Rabbit pAb (A2064)}

Immunohistochemistry - β-Catenin Rabbit pAb (A2064)

Immunohistochemistry analysis of β-Catenin in paraffin-embedded human breast cancer using β-Catenin Rabbit pAb (A2064) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - β-Catenin Rabbit pAb (A2064)}

Immunohistochemistry - β-Catenin Rabbit pAb (A2064)

Immunohistochemistry analysis of β-Catenin in paraffin-embedded mouse pancreas using β-Catenin Rabbit pAb (A2064) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - β-Catenin Rabbit pAb (A2064)}

Immunofluorescence - β-Catenin Rabbit pAb (A2064)

Immunofluorescence analysis of HeLa cells using β-Catenin Rabbit pAb (A2064) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on CTNNB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CTNNB1. (Distance between topics and target gene indicate popularity.) CTNNB1

* Data provided by citexs.com, for reference only.

Publishing research using A2064? Please let us know so that we can cite the reference in this datasheet.

Antibodies (16)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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