Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CPT1B Rabbit pAb (A6796)

Review (1) Publications (11) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CPT1B Rabbit pAb (A6796)

Western blot analysis of lysates from Rat skeletal muscle, using CPT1B Rabbit pAb (A6796) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CPT1B Rabbit pAb (A6796)

Immunohistochemistry analysis of CPT1B in paraffin-embedded human colon using CPT1B Rabbit pAb (A6796) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CPT1B Rabbit pAb (A6796)

Immunohistochemistry analysis of CPT1B in paraffin-embedded mouse heart using CPT1B Rabbit pAb (A6796) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for CPT1B Rabbit pAb(A6796)

You may also interested in:

Overview

Product name CPT1B Rabbit pAb
Catalog No. A6796
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1).
Sequence SYQVAKALADDVELYCFQFLPFGKGLIKKCRTSPDAFVQIALQLAHFRDRGKFCLTYEASMTRMFREGRTETVRSCTSESTAFVQAMMEGSHTKADLRDLFQKAAKKHQNMYRLAMTGAGIDRHLFCLYLVSKYLGVSSPFLAEVLSEPWRLSTSQIPQSQIRMFDPEQHPNHLGAGGGFGPVADDGYGVSYMIAGENTIFFHISSKFSSSETNAQRFGNHIRKALLDIADLFQVPKAYS
Gene ID 1375
Swiss prot Q92523
Synonyms CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1; CPT1B
Calculated MW 88kDa
Observed MW 88kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Rat skeletal muscle
Cellular location Mitochondrion outer membrane, Multi-pass membrane protein
Customer validation

WB (Mouse brown fat cellsHomo sapiens, Mus musculus, Homo sapiens, Macaca mulatta, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CPT1B Rabbit pAb images

ABclonal:Western blot - CPT1B Rabbit pAb (A6796)}

Western blot - CPT1B Rabbit pAb (A6796)

Western blot analysis of lysates from Rat skeletal muscle, using CPT1B Rabbit pAb (A6796) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CPT1B Rabbit pAb (A6796)}

Immunohistochemistry - CPT1B Rabbit pAb (A6796)

Immunohistochemistry analysis of CPT1B in paraffin-embedded human colon using CPT1B Rabbit pAb (A6796) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CPT1B Rabbit pAb (A6796)}

Immunohistochemistry - CPT1B Rabbit pAb (A6796)

Immunohistochemistry analysis of CPT1B in paraffin-embedded mouse heart using CPT1B Rabbit pAb (A6796) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Submit an review for A6796

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A6796 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on CPT1B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CPT1B. (Distance between topics and target gene indicate popularity.) CPT1B

* Data provided by citexs.com, for reference only.

Publishing research using A6796? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order