Summary
Catalog No:A10098
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - COX4I1 antibody (A10098)

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Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

ImmunogenA synthetic peptide of human COX4I1
SequenceEmail for sequence
Gene ID1327
Swiss ProtP13073
SynonymsCOXIV-1, COX4, COX4-1, COXIV, COXIV-1
Calculated MW19kDa
Observed MW17kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, ICC, IP, FC
Recommended DilutionWB 1:1000 - 1:4000
IHC 1:50 - 1:200
ICC 1:50 - 1:200
IP 1:20 - 1:50
FC 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    immunocytochemistry    Immunoprecipitation    Flow Cytometry    
Positive samplesLO2, U-87MG, MCF-7, K-562, Jurkat, Mouse skeletal muscle, Mouse kidney, Mouse heart, Rat brain
Cellular LocationMitochondrion inner membrane
Customer Validation

WB (Human)

COX4I1 Monoclonal Antibody images

ABclonal:Western blot - COX4I1 antibody (A10098)

Western blot - COX4I1 antibody (A10098)

Western blot analysis of extracts of various cell lines, using COX4I1 antibody (A10098) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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