Summary
Catalog No:A1018
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CNP antibody (A1018)

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Background

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 152-421 of human CNP (NP_149124.3).
SequenceTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPT
Gene ID1267
Swiss ProtP09543
SynonymsCNP1
Calculated MW45kDa/47kDa
Observed MW48kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHepG2, Rat brain, 293T, Mouse brain
Cellular LocationLipid-anchor, Melanosome, Membrane
Customer Validation

WB (Human)

CNP Polyclonal Antibody images

ABclonal:Western blot - CNP antibody (A1018)

Western blot - CNP antibody (A1018)

Western blot analysis of extracts of various cell lines, using CNP antibody (A1018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 15s.

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