Summary
Catalog No:A6259
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CHAF1B antibody (A6259)
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)
ABclonal:Immunofluorescence - CHAF1B antibody (A6259)

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Background

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
SequenceSRKNLKRPIAHLPCPGKATLAVRCCPVYFELRPVVETGVELMSLPYRLVFAVASEDSVLLYDTQQSFPFGYVSNIHYHTLSDISWSSDGAFLAISSTDGYCSFVTFEKDELGIPLKEKPVLNMRTPDTAKKTKSQTHRGSSPGPRPVEGTPASRTQDPSSPGTTPPQARQAPAPTVIRDPPSITPAVKSPLPGPSEEKTLQPSSQNTKAHPSRRVTLNTLQAWSKTTPRRINLTPLKTDTPPSSVPTSVISTPST
Gene ID8208
Swiss ProtQ13112
SynonymsCAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Calculated MW61kDa
Observed MW70kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (Mouse)

CHAF1B Polyclonal Antibody images

ABclonal:Western blot - CHAF1B antibody (A6259)

Western blot - CHAF1B antibody (A6259)

Western blot analysis of extracts of various cell lines, using CHAF1B antibody (A6259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)

Immunohistochemistry - CHAF1B antibody (A6259)

Immunohistochemistry of paraffin-embedded mouse kidney using CHAF1B antibody (A6259) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)

Immunohistochemistry - CHAF1B antibody (A6259)

Immunohistochemistry of paraffin-embedded rat brain using CHAF1B antibody (A6259) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)

Immunohistochemistry - CHAF1B antibody (A6259)

Immunohistochemistry of paraffin-embedded rat spleen using CHAF1B antibody (A6259) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)

Immunohistochemistry - CHAF1B antibody (A6259)

Immunohistochemistry of paraffin-embedded rat fallopian tube using CHAF1B antibody (A6259) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)

Immunohistochemistry - CHAF1B antibody (A6259)

Immunohistochemistry of paraffin-embedded human tonsil using CHAF1B antibody (A6259) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CHAF1B antibody (A6259)

Immunohistochemistry - CHAF1B antibody (A6259)

Immunohistochemistry of paraffin-embedded mouse spleen using CHAF1B antibody (A6259) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CHAF1B antibody (A6259)

Immunofluorescence - CHAF1B antibody (A6259)

Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody (A6259). Blue: DAPI for nuclear staining.

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