Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CDKN1B/p27KIP1 (A11800)

Review (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - CDKN1B/p27KIP1 (A11800)

Western blot analysis of extracts of various cell lines, using p27 KIP 1 antibody (A11800) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for CDKN1B/p27KIP1(A11800)

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Overview

Product name CDKN1B/p27KIP1
Catalog No. A11800
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).

Immunogen information

Immunogen Recombinant protein of human p27 KIP 1
Sequence Email for sequence
Gene ID 1027
Swiss prot P46527
Synonyms KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Calculated MW 22kDa
Observed MW 22kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Mouse lung
Cellular location Cytoplasm, Endosome, Nucleus
Customer validation

WB (Human breast cancer)

Research Area

CDKN1B/p27KIP1 images

ABclonal:Western blot - CDKN1B/p27KIP1 (A11800)}

Western blot - CDKN1B/p27KIP1 (A11800)

Western blot analysis of extracts of various cell lines, using p27 KIP 1 antibody (A11800) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDKN1B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDKN1B. (Distance between topics and target gene indicate popularity.) CDKN1B

* Data provided by citexs.com, for reference only.

Publishing research using A11800? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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