Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CDKN1A/p21CIP1 Rabbit pAb (A1483)

Reviews (3) Publications (61) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of lysates from MCF7 cells, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of various lysates, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)
Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)
Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)

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Overview

Product name CDKN1A/p21CIP1 Rabbit pAb
Catalog No. A1483
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).
Sequence MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP
Gene ID 1026
Swiss prot P38936
Synonyms P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; CDKN1A/p21CIP1
Calculated MW 18kDa
Observed MW 18kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples MCF7, Mouse large intestine, Mouse testis, Rat lung
Cellular location Cytoplasm, Nucleus
Customer validation

WB (rat cells, P19, P21Homo sapiens, Homo sapiens, Mus musculus, Sanghuangporus vaninii, Rattus norvegicus)

ChIP (Rattus norvegicus)

IF (Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus)

IHC (Homo sapiens, Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CDKN1A/p21CIP1 Rabbit pAb images

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of lysates from MCF7 cells, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of various lysates, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for A1483

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on CDKN1A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDKN1A. (Distance between topics and target gene indicate popularity.) CDKN1A

* Data provided by citexs.com, for reference only.

Publishing research using A1483? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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