Summary
Catalog No:A0016
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CDK4 antibody (A0016)

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Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Immunogen information

ImmunogenRecombinant protein of human CDK4
SequenceEmail for sequence
Gene ID1019
Swiss ProtP11802
SynonymsCMM3, PSK-J3
Calculated MW20kDa/33kDa
Observed MW34kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:1000
IHC 1:20 - 1:50
Storage BufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-431, HeLa, NIH/3T3, Mouse thymus
Cellular LocationCytoplasm, Membrane, Nucleus
Customer Validation

WB (Human)

CDK4 Monoclonal Antibody images

ABclonal:Western blot - CDK4 antibody (A0016)

Western blot - CDK4 antibody (A0016)

Western blot analysis of extracts of various cell lines, using CDK4 antibody (A0016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.

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