Summary
Catalog No:A0030
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CDK1 antibody (A0030)

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Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenRecombinant protein of human CDK1
SequenceEmail for sequence
Gene ID983
Swiss ProtP06493
SynonymsCDC2, CDC28A, P34CDC2
Calculated MW27kDa/34kDa
Observed MW37kDa

Applications

ReactivityHuman, Rat
Tested applicationsWB, ICC
Recommended DilutionWB 1:100 - 1:200
ICC 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    immunocytochemistry    
Positive samplesJurkat, A-549, K-562, HeLa, MCF-7, A-431, NIH/3T3, Rat thymus
Cellular LocationCytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer Validation

WB (Human)

CDK1 Monoclonal Antibody images

ABclonal:Western blot - CDK1 antibody (A0030)

Western blot - CDK1 antibody (A0030)

Western blot analysis of extracts of various cell lines, using CDK1 antibody (A0030) at 1:100 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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