Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CDK1 Mouse mAb (A0030)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CDK1 Mouse mAb (A0030)

Western blot analysis of various lysates using CDK1 Mouse mAb (A0030) at 1:100 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name CDK1 Mouse mAb
Catalog No. A0030
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant protein of human CDK1
Sequence Email for sequence
Gene ID 983
Swiss prot P06493
Synonyms CDC2; CDC28A; P34CDC2; CDK1
Calculated MW 34kDa
Observed MW 37kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:200
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Jurkat, A-549, K-562, HeLa, MCF7, A-431, NIH/3T3, Rat thymus
Cellular location Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CDK1 Mouse mAb images

ABclonal:Western blot - CDK1 Mouse mAb (A0030)}

Western blot - CDK1 Mouse mAb (A0030)

Western blot analysis of various lysates using CDK1 Mouse mAb (A0030) at 1:100 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CDK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CDK1. (Distance between topics and target gene indicate popularity.) CDK1

* Data provided by citexs.com, for reference only.

Publishing research using A0030? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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