Summary
Catalog No:A0112
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CCNE1 antibody (A0112)

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Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Immunogen information

ImmunogenA synthetic peptide of human CCNE1
SequenceEmail for sequence
Gene ID898
Swiss ProtP24864
SynonymsCCNE, pCCNE1
Calculated MW41kDa/45kDa/47kDa
Observed MW50kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, FC
Recommended DilutionWB 1:500 - 1:1000
IHC 1:20 - 1:50
FC 1:20 - 1:50
Storage BufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Flow Cytometry    
Positive samplesMouse lung
Cellular LocationNucleus
Customer Validation

WB (H460,A549, HeLa, Rat, Human)

IP (Rat)

CCNE1 Polyclonal Antibody images

ABclonal:Western blot - CCNE1 antibody (A0112)

Western blot - CCNE1 antibody (A0112)

Western blot analysis of extracts of mouse lung, using CCNE1 antibody (A0112).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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