Summary
Catalog No:A10757
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CCND1 antibody (A10757)
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)
ABclonal:Immunofluorescence - CCND1 antibody (A10757)
ABclonal:Immunofluorescence - CCND1 antibody (A10757)

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Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

Immunogen information

ImmunogenRecombinant protein of human CCND1
SequenceEmail for sequence
Gene ID595
Swiss ProtP24385
SynonymsBCL1, D11S287E, PRAD1, U21B31
Calculated MW33kDa
Observed MW34kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:1000 - 1:5000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationCytoplasm, Membrane, Nucleus
Customer Validation

WB (Human)

CCND1 Monoclonal Antibody images

ABclonal:Western blot - CCND1 antibody (A10757)

Western blot - CCND1 antibody (A10757)

Western blot analysis of extracts of various cell lines, using CCND1 antibody (A10757).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)

Immunohistochemistry - CCND1 antibody (A10757)

Immunohistochemistry of paraffin-embedded mouse liver using CCND1 antibody (A10757) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)

Immunohistochemistry - CCND1 antibody (A10757)

Immunohistochemistry of paraffin-embedded human tonsil using CCND1 antibody (A10757) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)

Immunohistochemistry - CCND1 antibody (A10757)

Immunohistochemistry of paraffin-embedded human liver cancer using CCND1 antibody (A10757) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CCND1 antibody (A10757)

Immunohistochemistry - CCND1 antibody (A10757)

Immunohistochemistry of paraffin-embedded human lung using CCND1 antibody (A10757) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CCND1 antibody (A10757)

Immunofluorescence - CCND1 antibody (A10757)

Immunofluorescence analysis of PC-12 cells using CCND1 antibody (A10757).
ABclonal:Immunofluorescence - CCND1 antibody (A10757)

Immunofluorescence - CCND1 antibody (A10757)

Immunofluorescence analysis of N2A cells using CCND1 antibody (A10757).

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