Summary
Catalog No:A3351
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CCNB2 antibody (A3351)

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Background

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-100 of human CCNB2 (NP_004692.1).
SequenceMALLRRPTVSSDLENIDTGVNSKVKSHVTIRRTVLEEIGNRVTTRAAQVAKKAQNTKVPVQPTKTTNVNKQLKPTASVKPVQMEKLAPKGPSPTPEDVSM
Gene ID9133
Swiss ProtO95067
SynonymsHsT17299
Calculated MW45kDa
Observed MW50kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, SW480, DU145, A-431, K-562, Mouse thymus
Cellular Location
Customer Validation

WB (Rat)

IP (Rat)

CCNB2 Polyclonal Antibody images

ABclonal:Western blot - CCNB2 antibody (A3351)

Western blot - CCNB2 antibody (A3351)

Western blot analysis of extracts of various cell lines, using CCNB2 antibody (A3351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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