Summary
Catalog No:A7632
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CCNA2 antibody (A7632)
ABclonal:Immunohistochemistry  - CCNA2 antibody (A7632)
ABclonal:Immunohistochemistry  - CCNA2 antibody (A7632)

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Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Immunogen information

ImmunogenA synthetic peptide of human CCNA2
SequenceEmail for sequence
Gene ID890
Swiss ProtP20248
SynonymsCCN1, CCNA
Calculated MW48kDa
Observed MW49kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (Rat)

IP (Rat)

CCNA2 Polyclonal Antibody images

ABclonal:Western blot - CCNA2 antibody (A7632)

Western blot - CCNA2 antibody (A7632)

Western blot analysis of extracts of various cell lines, using CCNA2 antibody (A7632) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry  - CCNA2 antibody (A7632)

Immunohistochemistry - CCNA2 antibody (A7632)

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using CCNA2 antibody (A7632) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CCNA2 antibody (A7632)

Immunohistochemistry - CCNA2 antibody (A7632)

Immunohistochemistry of paraffin-embedded mouse kidney using CCNA2 antibody (A7632) at dilution of 1:100 (40x lens).

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