Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CCAR2 Mouse mAb (A9920)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - CCAR2 Mouse mAb (A9920)

Western blot analysis of various lysates using CCAR2 Mouse mAb (A9920) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

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Overview

Product name CCAR2 Mouse mAb
Catalog No. A9920
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Enables RNA polymerase II complex binding activity and enzyme inhibitor activity. Involved in several processes, including regulation of cellular protein metabolic process; regulation of signal transduction; and regulation of transcription, DNA-templated. Located in several cellular components, including mitochondrial matrix; nucleoplasm; and spindle. Part of DBIRD complex.

Immunogen information

Immunogen Recombinant protein of human CCAR2
Sequence Email for sequence
Gene ID 57805
Swiss prot Q8N163
Synonyms DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967; CCAR2
Calculated MW 103kDa
Observed MW 140kDa

Applications

Reactivity Human, Mouse, Rat, Monkey
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:200 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples COS-7, Jurkat, A-549, HeLa, SH-SY5Y, LO2, HT-1080
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

CCAR2 Mouse mAb images

ABclonal:Western blot - CCAR2 Mouse mAb (A9920)}

Western blot - CCAR2 Mouse mAb (A9920)

Western blot analysis of various lysates using CCAR2 Mouse mAb (A9920) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCAR2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCAR2. (Distance between topics and target gene indicate popularity.) CCAR2

* Data provided by citexs.com, for reference only.

Publishing research using A9920? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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