Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Caspase-9 Rabbit pAb (A2636)

Review (1) Publications (49) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of various lysates using Caspase-9 Rabbit pAb (A2636) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of lysates from Jurkat cells, using Caspase-9 Rabbit pAb (A2636) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of Caspase-9 in paraffin-embedded rat heart using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of Caspase-9 in paraffin-embedded human lung cancer using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of Caspase-9 in paraffin-embedded mouse kidney using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Caspase-9 Rabbit pAb (A2636)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Caspase-9 Rabbit pAb(A2636)

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Overview

Product name Caspase-9 Rabbit pAb
Catalog No. A2636
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2).
Sequence VGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS
Gene ID 842
Swiss prot P55211
Synonyms MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; Caspase-9
Calculated MW 46kDa
Observed MW 36kDa/46kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Jurkat, K-562, Mouse liver
Cellular location apoptosome, cytoplasm, cytosol, mitochondrion, nucleus
Customer validation

WB (Human uterine stromal cellsHomo sapiens, Mus musculus, Homo sapiens, Giardia duodenalis, Holothuroidea, Apocynum venetum leaf, Sus scrofa, gga, Rattus norvegicus, Capra hircus)

RT-qPCR (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Caspase-9 Rabbit pAb images

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)}

Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of various lysates using Caspase-9 Rabbit pAb (A2636) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)}

Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of lysates from Jurkat cells, using Caspase-9 Rabbit pAb (A2636) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)}

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of Caspase-9 in paraffin-embedded rat heart using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)}

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of Caspase-9 in paraffin-embedded human lung cancer using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)}

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of Caspase-9 in paraffin-embedded mouse kidney using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Caspase-9 Rabbit pAb (A2636)}

Immunofluorescence - Caspase-9 Rabbit pAb (A2636)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on CASP9. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CASP9. (Distance between topics and target gene indicate popularity.) CASP9

* Data provided by citexs.com, for reference only.

Publishing research using A2636? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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