Summary
Catalog No:A2636
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CASP9 antibody (A2636)
ABclonal:Immunohistochemistry  - CASP9 Antibody (A2636)
ABclonal:Immunohistochemistry  - CASP9 Antibody (A2636)
ABclonal:Immunofluorescence - CASP9 antibody (A2636)

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Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Immunogen information

ImmunogenRecombinant protein of human CASP9
SequenceEmail for sequence
Gene ID842
Swiss ProtP55211
SynonymsAPAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
Calculated MW17kDa/30kDa/36kDa/46kDa
Observed MW46kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:200 - 1:500
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular Location
Customer Validation

WB (Human uterine stromal cells, Mouse)

CASP9 Polyclonal Antibody images

ABclonal:Western blot - CASP9 antibody (A2636)

Western blot - CASP9 antibody (A2636)

Western blot analysis of extracts of various cell lines, using CASP9 antibody (A2636) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry  - CASP9 Antibody (A2636)

Immunohistochemistry - CASP9 Antibody (A2636)

Immunohistochemistry of paraffin-embedded rat lung using CASP9 Antibody (A2636) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - CASP9 Antibody (A2636)

Immunohistochemistry - CASP9 Antibody (A2636)

Immunohistochemistry of paraffin-embedded rat brain using CASP9 Antibody (A2636) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CASP9 antibody (A2636)

Immunofluorescence - CASP9 antibody (A2636)

Immunofluorescence analysis of A549 cells using CASP9 antibody (A2636).

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