Summary
Catalog No:A0215
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CASP8 antibody (A0215)

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Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-479 of human CASP8 (NP_001073593.1).
SequenceMDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGAL
Gene ID841
Swiss ProtQ14790
SynonymsALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
Calculated MW25-32kDa/45- 61kDa
Observed MW17kDa/44kDa/60kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRaji, HL-60
Cellular LocationCytoplasm
Customer Validation

WB (N9, Mouse)

CASP8 Polyclonal Antibody images

ABclonal:Western blot - CASP8 antibody (A0215)

Western blot - CASP8 antibody (A0215)

Western blot analysis of extracts of various cell lines, using CASP8 antibody (A0215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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