Summary
Catalog No:A6495
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CASP4 antibody (A6495)

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Background

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Immunogen information

ImmunogenRecombinant protein of mouse CASP4
SequenceEmail for sequence
Gene ID12363
Swiss Prot
SynonymsICE(rel)II, ICEREL-II, ICH-2, Mih1, Mih1/TX, TX
Calculated MW13kDa/18kDa/30kDa/36kDa/43kDa
Observed MW43-48kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, HeLa
Cellular LocationEndoplasmic reticulum membrane, Mitochondrion
Customer Validation

WB (Mouse)

CASP4 Polyclonal Antibody images

ABclonal:Western blot - CASP4 antibody (A6495)

Western blot - CASP4 antibody (A6495)

Western blot analysis of extracts of various cell lines, using CASP4 antibody (A6495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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