Summary
Catalog No:A2156
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - CASP3 antibody (A2156)

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Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 55-160 of human CASP3 (NP_004337.2).
SequenceFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
Gene ID836
Swiss ProtP42574
SynonymsCPP32, CPP32B, SCA-1
Calculated MW31kDa
Observed MW17kDa, 35kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:1000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesJurkat
Cellular LocationCytoplasm
Customer Validation

WB (HeLa, Human uterine stromal cells, B6, NMuMG, P. notoginseng, Human, Mouse, Rat, Pig)

IHC (Mouse kidney, Rat brain)

CASP3 Polyclonal Antibody images

ABclonal:Western blot - CASP3 antibody (A2156)

Western blot - CASP3 antibody (A2156)

Western blot analysis of extracts of Jurkat cells, using CASP3 antibody (A2156) at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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