Summary
Catalog No:A0212
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - BRCA1 antibody (A0212)
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)
ABclonal:Immunofluorescence - BRCA1 antibody (A0212)

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Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Immunogen information

ImmunogenA synthetic peptide of human BRCA1
SequenceEmail for sequence
Gene ID672
Swiss ProtP38398
SynonymsBRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
Calculated MW7kDa/78-85kDa/202-210kDa
Observed MW85kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationChromosome, Cytoplasm, Cytoplasm, Nucleus
Customer Validation

WB (Mouse adipose-derived stem cells, Human)

BRCA1 Polyclonal Antibody images

ABclonal:Western blot - BRCA1 antibody (A0212)

Western blot - BRCA1 antibody (A0212)

Western blot analysis of extracts of various cell lines, using BRCA1 antibody (A0212).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)

Immunohistochemistry - BRCA1 antibody (A0212)

Immunohistochemistry of paraffin-embedded human prostate using BRCA1 antibody (A0212) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)

Immunohistochemistry - BRCA1 antibody (A0212)

Immunohistochemistry of paraffin-embedded mouse brain using BRCA1 antibody (A0212) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)

Immunohistochemistry - BRCA1 antibody (A0212)

Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody (A0212) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)

Immunohistochemistry - BRCA1 antibody (A0212)

Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody (A0212) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - BRCA1 antibody (A0212)

Immunohistochemistry - BRCA1 antibody (A0212)

Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody (A0212) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - BRCA1 antibody (A0212)

Immunofluorescence - BRCA1 antibody (A0212)

Immunofluorescence analysis of A549 cells using BRCA1 antibody (A0212).

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