Summary
Catalog No:A7353
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - BECN1 antibody (A7353)

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Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-280 of human BECN1 (NP_003757.1).
SequenceMEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMR
Gene ID8678
Swiss ProtQ14457
SynonymsATG6, beclin1, VPS30
Calculated MW51kDa
Observed MW60kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesU-87MG, HeLa, MCF7, Mouse spleen, Rat ovary, Rat lung
Cellular LocationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane
Customer Validation

WB (C2C12, A549,Mouse kidney, U2OS,Saos-2, Human Isikawa , NPCs, Pig)

IHC (A549,Mouse kidney)

BECN1 Polyclonal Antibody images

ABclonal:Western blot - BECN1 antibody (A7353)

Western blot - BECN1 antibody (A7353)

Western blot analysis of extracts of various cell lines, using BECN1 antibody (A7353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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