Summary
Catalog No:A0208
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - BCL2 antibody (A0208)
ABclonal:Immunohistochemistry  - BCL2 antibody (A0208)
ABclonal:Immunohistochemistry  - BCL2 antibody (A0208)

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Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Immunogen information

ImmunogenA synthetic peptide of human BCL2
SequenceEmail for sequence
Gene ID596
Swiss ProtP10415
SynonymsBcl-2, PPP1R50
Calculated MW22kDa/26kDa
Observed MW26kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse liver, Rat liver
Cellular LocationEndoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
Customer Validation

WB (Human skin T cell lymphoma, CAL27, Hut102, HCT116, MCF-7,MD-MBA-231, B6, Rat, Human, Mouse, Rattus norvegicus)

BCL2 Polyclonal Antibody images

ABclonal:Western blot - BCL2 antibody (A0208)

Western blot - BCL2 antibody (A0208)

Western blot analysis of extracts of various cell lines, using BCL2 antibody (A0208) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry  - BCL2 antibody (A0208)

Immunohistochemistry - BCL2 antibody (A0208)

Immunohistochemistry of paraffin-embedded human liver cancer using BCL2 antibody (A0208) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - BCL2 antibody (A0208)

Immunohistochemistry - BCL2 antibody (A0208)

Immunohistochemistry of paraffin-embedded human gastric cancer using BCL2 antibody (A0208) at dilution of 1:100 (40x lens).

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