Summary
Catalog No:A0207
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - BAX antibody (A0207)
ABclonal:Immunohistochemistry  - Bax antibody (A0207)

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Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAX (NP_620116.1).
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID581
Swiss ProtQ07812
SynonymsBCL2L4
Calculated MW4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW21kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples
Cellular LocationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Customer Validation

WB (Human skin T cell lymphoma, Hut102, HCT116, Rat)

BAX Polyclonal Antibody images

ABclonal:Western blot - BAX antibody (A0207)

Western blot - BAX antibody (A0207)

Western blot analysis of extracts of Raji cells, using BAX antibody (A0207) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry  - Bax antibody (A0207)

Immunohistochemistry - Bax antibody (A0207)

Immunohistochemistry of paraffin-embedded mouse lung using Bax antibody (A0207) at dilution of 1:100 (40x lens).

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