Products / Catalog Antibodies / Methylated Antibodies

Asymmetric DiMethyl-Histone H4-R3 pAb

Summary
Catalog No:A2376
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)
ABclonal:Dot-blot - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

ImmunogenA synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
SequenceEmail for sequence
Gene ID8370
Swiss ProtP62805
SynonymsFO108, H4, H4/n, H4F2, H4FN, HIST2H4
Calculated MW11kDa
Observed MW13kDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationChromosome, Nucleus
Customer Validation

WB (Human)

ChIP (Human)

Asymmetric DiMethyl-Histone H4-R3 pAb images

ABclonal:Western blot - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Western blot - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H4-R3 antibody (A2376).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Dot-blot - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Dot-blot - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H4-R3 antibody (A2376).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry of paraffin-embedded rat brain using Asymmetric DiMethyl-Histone H4-R3 antibody (A2376) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry of paraffin-embedded rat kidney using Asymmetric DiMethyl-Histone H4-R3 antibody (A2376) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H4-R3 antibody (A2376) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunohistochemistry of paraffin-embedded human rectal cancer using Asymmetric DiMethyl-Histone H4-R3 antibody (A2376) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunofluorescence - Asymmetric DiMethyl-Histone H4-R3 antibody (A2376)

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H4-R3 antibody (A2376). Blue: DAPI for nuclear staining.

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