Products / Catalog Antibodies / Methylated Antibodies

Asymmetric DiMethyl-Histone H3-R17 pAb

Summary
Catalog No:A2421
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)
ABclonal:Dot-blot - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

ImmunogenA synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
SequenceEmail for sequence
Gene ID8356
Swiss ProtP68431
SynonymsH3/j, H3FJ
Calculated MW15kDa
Observed MW17kDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationChromosome, Nucleus
Customer Validation

WB (Hippocampus, Mouse)

Asymmetric DiMethyl-Histone H3-R17 pAb images

ABclonal:Western blot - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Western blot - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Dot-blot - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Dot-blot - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry of paraffin-embedded rat lung using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry of paraffin-embedded human kidney cancer using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunohistochemistry of paraffin-embedded mouse lung using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R17 antibody (A2421)

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421). Blue: DAPI for nuclear staining.

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