Summary
Catalog No:A11012
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Acetyl-TP53-K370 antibody (A11012)
ABclonal:Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)
ABclonal:Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)
ABclonal:Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)

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Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

ImmunogenA specific peptide of human Acetyl-TP53-K370
SequenceEmail for sequence
Gene ID7157
Swiss ProtP04637
SynonymsBCC7, LFS1, P53, TRP53
Calculated MW23-43kDa
Observed MW53kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IF, IP
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body

Acetyl-TP53-K370 mAb images

ABclonal:Western blot - Acetyl-TP53-K370 antibody (A11012)

Western blot - Acetyl-TP53-K370 antibody (A11012)

Western blot analysis of extracts of various cell lines, using Acetyl-TP53-K370 antibody (A11012).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)

Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)

Immunofluorescence analysis of PANC-1 cells using Acetyl-TP53-K370 antibody (A11012).
ABclonal:Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)

Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)

Immunofluorescence analysis of HeLa cells using Acetyl-TP53-K370 antibody (A11012).
ABclonal:Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)

Immunofluorescence - Acetyl-TP53-K370 antibody (A11012)

Immunofluorescence analysis of HepG2 cells using Acetyl-TP53-K370 antibody (A11012).

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