Summary
Catalog No:A7258
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Acetyl-Histone H4-K8 antibody (A7258)
ABclonal:Immunofluorescence - Acetyl-Histone H4-K8 antibody (A7258)
ABclonal:Chromatin immunoprecipitation - H4K8ac antibody (A7258)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

ImmunogenA synthetic peptide of human Acetyl-Histone H4-K8
SequenceEmail for sequence
Gene ID8370
Swiss ProtP62805
SynonymsFO108, H4, H4/n, H4F2, H4FN, HIST2H4
Calculated MW11kDa
Observed MW11kDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWB, IHC, IF, IP, ChIP, ChIPseq
Recommended DilutionWB 1:1000 - 1:3000
IHC 1:200 - 1:500
IF 1:500 - 1:1000
IP 1:200 - 1:500
CHIP 1:20 - 1:50
CHIPseq 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationChromosome, Nucleus

Acetyl-Histone H4-K8 pAb images

ABclonal:Western blot - Acetyl-Histone H4-K8 antibody (A7258)

Western blot - Acetyl-Histone H4-K8 antibody (A7258)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (A7258) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K8 antibody (A7258)

Immunofluorescence - Acetyl-Histone H4-K8 antibody (A7258)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (A7258). Blue: DAPI for nuclear staining.
ABclonal:Chromatin immunoprecipitation - H4K8ac antibody (A7258)

Chromatin immunoprecipitation - H4K8ac antibody (A7258)

Chromatin immunoprecipitation analysis extracts of 293 cell line, using H4K8ac antibody (A7258) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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