Summary
Catalog No:A15233
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Acetyl-Histone H4-K5 antibody (A15233)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

ImmunogenA synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
SequenceGRGKG
Gene ID8370
Swiss ProtP62805
SynonymsFO108, H4, H4/n, H4F2, H4FN, HIST2H4
Calculated MW11kDa
Observed MW11kDa

Applications

ReactivityHuman, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, C6
Cellular LocationChromosome, Nucleus

Acetyl-Histone H4-K5 pAb images

ABclonal:Western blot - Acetyl-Histone H4-K5 antibody (A15233)

Western blot - Acetyl-Histone H4-K5 antibody (A15233)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (A15233) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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