Summary
Catalog No:A11015
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)
ABclonal:Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Immunogen information

ImmunogenA specific peptide of human Acetyl-Histone H4-K5
SequenceEmail for sequence
Gene ID8366
Swiss ProtP62805
SynonymsH4/I, H4FI
Calculated MW11kDa
Observed MW11kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples
Cellular LocationChromosome, Nucleus

Acetyl-Histone H4-K5 mAb images

ABclonal:Western blot - Acetyl-Histone H4-K5 antibody (A11015)

Western blot - Acetyl-Histone H4-K5 antibody (A11015)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (A11015).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H4-K5 antibody (A11015) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry of paraffin-embedded human tonsil using Acetyl-Histone H4-K5 antibody (A11015) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H4-K5 antibody (A11015) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K5 antibody (A11015) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry - Acetyl-Histone H4-K5 antibody (A11015)

Immunohistochemistry of paraffin-embedded mouse colon using Acetyl-Histone H4-K5 antibody (A11015) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)

Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)

Immunofluorescence analysis of CRC cells using Acetyl-Histone H4-K5 antibody (A11015).
ABclonal:Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)

Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K5 antibody (A11015).
ABclonal:Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)

Immunofluorescence - Acetyl-Histone H4-K5 antibody (A11015)

Immunofluorescence analysis of SH-SY-5Y cells using Acetyl-Histone H4-K5 antibody (A11015).

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