Summary
Catalog No:A7255
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Acetyl-Histone H3-K9 antibody (A7255)
ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 antibody (A7255)
ABclonal:Chromatin immunoprecipitation - H3K9ac antibody (A7255)

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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

ImmunogenA synthetic peptide of human Acetyl-Histone H3-K9
SequenceEmail for sequence
Gene ID8290
Swiss ProtQ16695
SynonymsH3.4, H3/g, H3FT, H3t
Calculated MW15kDa
Observed MW16kDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWB, IHC, IF, IP, ChIP, ChIPseq
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesBT-474, 293T, H3 protein
Cellular LocationChromosome, Nucleus

Acetyl-Histone H3-K9 pAb images

ABclonal:Western blot - Acetyl-Histone H3-K9 antibody (A7255)

Western blot - Acetyl-Histone H3-K9 antibody (A7255)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (A7255) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 antibody (A7255)

Immunofluorescence - Acetyl-Histone H3-K9 antibody (A7255)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (A7255). Blue: DAPI for nuclear staining.
ABclonal:Chromatin immunoprecipitation - H3K9ac antibody (A7255)

Chromatin immunoprecipitation - H3K9ac antibody (A7255)

Chromatin immunoprecipitation analysis extracts of 293 cell line, using H3K9ac antibody (A7255) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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