Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Publications (11) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Dot Blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (A7257) at 1:1000 dilution.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunohistochemistry analysis of Acetyl-Histone H3-K18 in paraffin-embedded rat brain using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunohistochemistry analysis of Acetyl-Histone H3-K18 in paraffin-embedded human gastric cancer using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunohistochemistry analysis of Acetyl-Histone H3-K18 in paraffin-embedded mouse brain using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb antibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb yantibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

You may also interested in:

Overview

Product name Acetyl-Histone H3-K18 Rabbit pAb
Catalog No. A7257
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic acetylated peptide around K18 of human H3 (NP_003520.1).
Sequence PRKQL
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J; Acetyl-Histone H3-K18
Calculated MW 15kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
  • DB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa+TSA
Cellular location Chromosome, Nucleus
Customer validation

WB (Mus musculus, Homo sapiens, Saccharomyces cerevisiae, Monascus ruber, Oryza sativa Japonica Group, Eurosta solidaginis)

CHIP (Homo sapiens)

ChIP (F. graminearum)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Acetyl-Histone H3-K18 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Western blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
ABclonal:Dot Blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Dot Blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (A7257) at 1:1000 dilution.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunohistochemistry analysis of Acetyl-Histone H3-K18 in paraffin-embedded rat brain using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunohistochemistry analysis of Acetyl-Histone H3-K18 in paraffin-embedded human gastric cancer using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunohistochemistry analysis of Acetyl-Histone H3-K18 in paraffin-embedded mouse brain using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb antibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)}

Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb yantibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Inquire About This Product

Submit your question about A7257 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on H3C12. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3C12. (Distance between topics and target gene indicate popularity.) H3C12

* Data provided by citexs.com, for reference only.

Publishing research using A7257? Please let us know so that we can cite the reference in this datasheet.

Antibodies (114)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order