Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

p16-ARC/ARC16/ARPC5 Rabbit mAb (A11317)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - p16-ARC/ARC16/ARPC5 Rabbit mAb (A11317)

Western blot analysis of extracts of various cell lines, using p16-ARC/ARC16/p16-ARC/ARC16/ARPC5 antibody (A11317).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name p16-ARC/ARC16/ARPC5 Rabbit mAb
Catalog No. A11317
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene.

Immunogen information

Immunogen Recombinant protein of human p16-ARC/ARC16/p16-ARC/ARC16/ARPC5
Sequence Email for sequence
Gene ID 10092
Swiss prot O15511
Synonyms ARC16; p16-Arc; dJ127C7.3; p16-ARC/ARC16/ARPC5
Calculated MW 16kDa
Observed MW 16kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples MCF7, SK-BR-3
Cellular location Cell projection, Cytoplasm, cytoskeleton

Research Area

p16-ARC/ARC16/ARPC5 Rabbit mAb images

ABclonal:Western blot - p16-ARC/ARC16/ARPC5 Rabbit mAb (A11317)}

Western blot - p16-ARC/ARC16/ARPC5 Rabbit mAb (A11317)

Western blot analysis of extracts of various cell lines, using p16-ARC/ARC16/p16-ARC/ARC16/ARPC5 antibody (A11317).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on ARPC5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ARPC5. (Distance between topics and target gene indicate popularity.) ARPC5

* Data provided by citexs.com, for reference only.

Publishing research using A11317? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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