Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Annexin A2 Rabbit pAb (A1572)

Review (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Annexin A2 Rabbit pAb (A1572)

Western blot analysis of various lysates using Annexin A2 Rabbit pAb (A1572) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Annexin A2 Rabbit pAb (A1572)

Western blot analysis of various lysates using Annexin A2 Rabbit pAb (A1572) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human liver cancer using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human liver using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse kidney using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse lung using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded rat heart using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Annexin A2 Rabbit pAb (A1572)

Immunofluorescence analysis of MCF7 cells using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Annexin A2 Rabbit pAb (A1572)

Immunofluorescence analysis of NIH/3T3 cells using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Annexin A2 Rabbit pAb(A1572)

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Overview

Product name Annexin A2 Rabbit pAb
Catalog No. A1572
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. Annexin A2 expression has been found to correlate with resistance to treatment against various cancer forms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human Annexin A2 (NP_004030.1).
Sequence MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
Gene ID 302
Swiss prot P07355
Synonyms P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270; Annexin A2
Calculated MW 39kDa
Observed MW 35kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples MCF7, LNCaP(negative), Mouse kidney, Rat kidney
Cellular location Melanosome, Secreted, basement membrane, extracellular matrix, extracellular space
Customer validation

WB (Huh7.5.1)

Documents

Certificate of Compliance

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Lot number

Research Area

Annexin A2 Rabbit pAb images

ABclonal:Western blot - Annexin A2 Rabbit pAb (A1572)}

Western blot - Annexin A2 Rabbit pAb (A1572)

Western blot analysis of various lysates using Annexin A2 Rabbit pAb (A1572) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Annexin A2 Rabbit pAb (A1572)}

Western blot - Annexin A2 Rabbit pAb (A1572)

Western blot analysis of various lysates using Annexin A2 Rabbit pAb (A1572) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)}

Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human liver cancer using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)}

Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human liver using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)}

Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse kidney using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)}

Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse lung using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)}

Immunohistochemistry - Annexin A2 Rabbit pAb (A1572)

Immunohistochemistry analysis of Annexin A2 in paraffin-embedded rat heart using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Annexin A2 Rabbit pAb (A1572)}

Immunofluorescence - Annexin A2 Rabbit pAb (A1572)

Immunofluorescence analysis of MCF7 cells using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Annexin A2 Rabbit pAb (A1572)}

Immunofluorescence - Annexin A2 Rabbit pAb (A1572)

Immunofluorescence analysis of NIH/3T3 cells using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on ANXA2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ANXA2. (Distance between topics and target gene indicate popularity.) ANXA2

* Data provided by citexs.com, for reference only.

Publishing research using A1572? Please let us know so that we can cite the reference in this datasheet.

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