Summary
Catalog No:AC006
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - β-actin antibody (AC006)

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Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-375 of human ACTB (NP_001092.1).
SequenceMDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERF
Gene ID60
Swiss ProtP60709
SynonymsBRWS1, PS1TP5BP1
Calculated MW41kDa
Observed MW42kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, 293T, A-431, Mouse lung, Mouse lung, Mouse spleen, Mouse kidney, Mouse thymus
Cellular LocationCytoplasm, cytoskeleton
Customer Validation

WB (C. gigas, Human)

ACTB Polyclonal Antibody images

ABclonal:Western blot - β-actin antibody (AC006)

Western blot - β-actin antibody (AC006)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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