Summary
Catalog No:AC026
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - β-actin antibody (AC026)
ABclonal:Western blot - β-actin antibody (AC026)
ABclonal:Immunohistochemistry  - β-actin antibody (AC026)
ABclonal:Immunohistochemistry  - β-actin antibody (AC026)
ABclonal:Immunohistochemistry  - β-actin antibody (AC026)

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Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen information

ImmunogenRecombinant protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss ProtP60709
SynonymsBRWS1, PS1TP5BP1
Calculated MW41kDa
Observed MW43kDa

Applications

ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
Tested applicationsWB, IHC
Recommended DilutionWB 1:50000 - 1:200000
IHC 1:200 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples
Cellular LocationCytoplasm, cytoskeleton
Customer Validation

WB (Pig lung, 293T, DF-1, Human, Clam)

ACTB Monoclonal Antibody images

ABclonal:Western blot - β-actin antibody (AC026)

Western blot - β-actin antibody (AC026)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - β-actin antibody (AC026)

Western blot - β-actin antibody (AC026)

Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry  - β-actin antibody (AC026)

Immunohistochemistry - β-actin antibody (AC026)

Immunohistochemistry of paraffin-embedded human breast cancer using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC026)

Immunohistochemistry - β-actin antibody (AC026)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC026)

Immunohistochemistry - β-actin antibody (AC026)

Immunohistochemistry of paraffin-embedded rat kidney using β-actin antibody (AC026) at dilution of 1:100 (20x lens).

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