Summary
Catalog No:AC004
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

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Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen information

ImmunogenFusion protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss ProtP60709
SynonymsBRWS1, PS1TP5BP1
Calculated MW41kDa
Observed MW42kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:10000 - 1:100000
IHC 1:100- 1:200
IF 1:50- 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationCytoplasm, cytoskeleton
Customer Validation

IHC (Mouse kidney, Rat kidney)

WB (Rat, Human, Mouse, Rat, Pig, Murine, Citrus sinensis, Pacific oyster, Tongue sole)

IF (Human)

IP (Human)

ACTB Monoclonal Antibody images

ABclonal:Western blot - β-actin antibody (AC004)

Western blot - β-actin antibody (AC004)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC004) at 1:80000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded rat liver using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded rat testis using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded rat spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded rat heart using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded human stomach using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry  - β-actin antibody (AC004)

Immunohistochemistry - β-actin antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse heart using β-actin antibody (AC004) at dilution of 1:500 (40x lens).

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