Summary
Catalog No:A2625
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - ACTA2 antibody (A2625)

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Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Immunogen information

ImmunogenA synthetic peptide of human ACTA2
SequenceEmail for sequence
Gene ID59
Swiss ProtP62736
SynonymsAAT6, ACTSA, MYMY5
Calculated MW42kDa
Observed MW42kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, ICC, FC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
ICC 1:50 - 1:200
FC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    immunocytochemistry    Flow Cytometry    
Positive samplesHeLa
Cellular LocationCytoplasm, cytoskeleton
Customer Validation

IF (pig lung)

WB (Mouse,Rat)

IHC (Rat)

ACTA2 Monoclonal Antibody images

ABclonal:Western blot - ACTA2 antibody (A2625)

Western blot - ACTA2 antibody (A2625)

Western blot analysis of extracts of HeLa cells, using ACTA2 antibody (A2625).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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